CST, 43640S, DNA Ligase III (F2A1H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LIG3. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, DNA Ligase III (F2A1H) Rabbit Monoclonal Antibody (CST #43640) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DNA Ligase III (F2A1H) Rabbit Monoclonal Antibody recognizes endogenous levels of total DNA ligase III protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human DNA ligase III protein. Background DNA ligase III/LIG3 is an ATP-dependent DNA ligase that functions in DNA repair. Four isoforms of LIG3 result from alternative splicing/initiation, including a mitochondrial-specific isoform essential for cell viability (1). Loss of nuclear LIG3 sensitizes resistant cells to PARP inhibitors and enhances PARP inhibitor efficacy in BRCA1-deficient mouse mammary tumors (2). In multiple myeloma (MM), LIG3 is crucial for repair of DNA double-stranded breaks (DSBs) via alternative-non-homologous end joining (Alt-NHEJ), and LIG3 inhibitors are being investigated as cancer therapeutics (3). Alternate Names DNA ligase 3; DNA ligase III; DNLI3; LIG2; LIG3; LIG3alpha; ligase II, DNA, ATP-dependent; ligase III, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG