CST, 43666S, ASCL1 (E7N9C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ASCL1. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Highly specific and rigorously validated in-house, ASCL1 (E7N9C) Rabbit Monoclonal Antibody (CST #43666) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity ASCL1 (E7N9C) Rabbit Monoclonal Antibody recognizes endogenous levels of total ASCL1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe108 of human ASCL1 protein. Background Achaete-scute homolog 1 (ASCL1), also known as MASH-1, is a basic helix-loop-helix (BHLH) transcription factor which plays an essential role in the differentiation of neuroendocrine cells and neural tissues (1-3). ASCL1 directly binds the E-box motif (5'-CANNTG-3') on promoters, with dimerization with other BHLH proteins required for efficient DNA binding (4). Acting as a pioneer transcription factor, ASCL1 also accesses closed chromatin, allowing other factors to promote transcription of neuronal genes and activate neural pathways (5). Research studies have shown that knockdown of the gene leads to inhibition of growth and induction of apoptosis in SCLC cells (6). Additionally, ASCL1 is overexpressed in both classic SCLC as well as NSCLC with neuroendocrine features, suggesting its role in the pathogenesis of those malignancies (7). ASCL1 plays a crucial role in promoting SCLC carcinogenesis by upregulating the expression of DLL3, a nonfunctioning Notch ligand, leading to inhibition of the Notch signaling pathway (8). Alternate Names achaete scute protein; achaete-scute complex homolog 1; achaete-scute complex homolog 1 (Drosophila); achaete-scute complex-like 1; achaete-scute family bHLH transcription factor 1; Achaete-scute homolog 1; ASCL1; ASH-1; ASH1; BHLHA46; Class A basic helix-loop-helix protein 46; HASH1; MASH1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG