CST, 43710T, CCL3/MIP-1 alpha (F3I3P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CCL3. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, CCL3/MIP-1 alpha (F3I3P) Rabbit Monoclonal Antibody (CST #43710) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CCL3/MIP-1 alpha (F3I3P) Rabbit Monoclonal Antibody recognizes endogenous levels of total CCL3/MIP-1α protein. This antibody does not cross-react with CCL4 or CCL18 proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro43 of human CCL3/MIP-1α protein. Background Chemokine (C-C motif) ligand 3 (CCL3), also known as macrophage inflammatory protein-1α (MIP-1α), is a 10 kDa member of the C-C or β family of chemokines that play key roles in immune surveillance, inflammation, and infection (1). CCL3 shares 68% amino acid identity with CCL4 (MIP-1β) and 94% amino acid identity with LD78β (1). CCL3 is produced by activated monocytes, activated macrophages, NK cells, and T cells (1). There are two receptors for CCL3: CCR1 and CCR5 (1). CCL3 inhibits R5 HIV-1 infection by competing with the virus for its co-receptor, CCR5 (1). CCL3 possesses hematopoietic stem cell inhibitory activity, suppressing their proliferation, but has been shown to promote proliferation of more mature progenitors (2-4). CCL3 has been implicated in several forms of leukemia, in which malignant cells may lose responsiveness to CCL3 or produce high levels of CCL3 (2). CCL3 polymorphisms are associated with risk for the development of rheumatoid arthritis and ulcerative colitis (5,6). CCL3 expression is upregulated in the brains of patients with Alzheimer's disease (AD) with dementia, and reduced expression of CCL3 is observed in AD patients resilient to dementia (7). Alternate Names C-C motif chemokine 3; C-C motif chemokine ligand 3; CCL3; G0/G1 switch regulatory protein 19-1; G0S19-1; LD78-alpha(4-69); LD78ALPHA; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; MIP-1-alpha(4-69); MIP1A; PAT 464.1; SCYA3; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 10 Source/Isotype: Rabbit IgG