CST, 43724T, Spermidine synthase (F5X6P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SRM. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Spermidine synthase (F5X6P) Rabbit Monoclonal Antibody (CST #43724) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Spermidine synthase (F5X6P) Rabbit Monoclonal Antibody recognizes endogenous levels of total spermidine synthase protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human spermidine synthase protein. Background Polyamines, including spermine, spermidine, and putrescine, are involved in many cellular processes, for example, cell proliferation, differentiation, apoptosis, the synthesis of proteins and nucleic acids, ion channel activity regulation, and the protection from oxidative injury (1). The cellular content of polyamines is tightly regulated; their biosynthesis is catalyzed by different enzymes including spermine synthase (SMS) and spermidine synthase (SRM), while the enzymes N1-acetylpolyamine oxidase (PAOX), spermidine/spermine N1-acetyltransferase 1 (SAT1), and spermine oxidase (SMOX) are involved in polyamine catabolism (1). SRM catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (2). In the brain, SRM is predominantly localized to neurons and neuropil with low expression in glial cells (3,4). Alternate Names PAPT; Putrescine aminopropyltransferase; SPDSY; SPEE; Spermidine synthase; spermidine synthase-1; SPS1; SRM; SRML1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 33 Source/Isotype: Rabbit IgG