CST, 43731T, LshCas13a/C2c2 (Leptotrichia shahii) (7D1-H7) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Cas13a/LshC2c2 bacteria. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, LshCas13a/C2c2 (Leptotrichia shahii) (7D1-H7) Mouse Monoclonal Antibody (CST #43731) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity LshCas13a/C2c2 ( Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of LshCas13a/C2c2 ( ) protein. Background CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cas13 proteins are members of the Class 2 CRISPR-Cas system. Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA/RNA interference rather than the multi-Cas protein complexes that belong to the Class 1 systems (2). Evolutionarily, CRISPR-Cas proteins act as RNA-guided antiviral and anti-plasmid that protect prokaryotes from phage-mediated infections. RNA-guided CRISPR-Cas proteins can act on both DNA and RNA. Cas13 proteins act as RNA-guided RNA nucleases, disrupting phage infection by RNA cleavage. The CRISPR-Cas system is being used for nuclease-based gene-editing. Since the Cas13 proteins act on RNA rather than DNA, Cas13 proteins, coupled with the appropriate guide RNA, can be used to target specific RNA substrates, potentially replacing or complementing RNA interference approaches that interfere with transcript levels (3). Several Cas13 bacterial orthologs have been characterized for CRISPR-mediated mammalian RNA editing, including LshCas13a/C2c2 and PspCas13b derived from and , respectively (4-6). Alternate Names c2c2; cas13a; Cas13a/LshC2c2; CS13A_LEPSD Specification REACTIVITY: All SENSITIVITY: Transfected Only MW (kDa): 166 Source/Isotype: Mouse IgG1