CST, 43781S, CHMP7 (F4P9J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CHMP7. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CHMP7 (F4P9J) Rabbit Monoclonal Antibody (CST #43781) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CHMP7 (F4P9J) Rabbit Monoclonal Antibody recognizes endogenous levels of total CHMP7 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro402 of human CHMP7 protein. Background Charged multivesicular body protein 7 (CHMP7) is a part of the endosomal sorting complex required for transport-III (ESCRT-III) (1,2). CHMP7 is typically found in the cytoplasm under homeostatic conditions, but it continually diffuses into the nucleus (1,2). To prevent accumulation, it is then actively exported back to the cytoplasm by exportin-1 (1,2). Upon completion of mitosis, CHMP7 helps to reform the nuclear envelope (3). CHMP7 can also regulate interactions between the endoplasmic reticulum and mitochondria (3). Micronuclei, often present in cancer cells with chromosomal instability, can be disrupted by CHMP7 accumulation and oligomerization, which occurs with increased intracellular reactive oxygen species (4). CHMP7 is also involved in amyotrophic lateral sclerosis (ALS) and may be a therapeutic target. Aberrant CHMP7 nuclear accumulation in neurons leads to nuclear pore damage and TDP-43 dysfunction (2,5). Alternate Names Charged multivesicular body protein 7; CHMP family, member 7; CHMP7; Chromatin-modifying protein 7; MGC29816 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 61 Source/Isotype: Rabbit IgG