CST, 43852W2, IRF-5 (E9I4Z) Rabbit Monoclonal Antibody (SignalFlex™ Pacific Blue™ Conjugate)

Monoclonal Antibody for studying IRF-5. Highly specific and rigorously validated in-house, IRF-5 (E9I4Z) Rabbit Monoclonal Antibody (SignalFlex^™ Pacific Blue^™ Conjugate) (CST #43852) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity IRF-5 (E9I4Z) Rabbit Monoclonal Antibody (SignalFlex™ Pacific Blue™ Conjugate) recognizes human IRF-5 protein and is also reactive with mouse IRF-5 protein; however, this antibody is not suggested for immunofluorescent analysis of human samples. Instead, IRF-5 (E7F9W) Rabbit mAb #76983 is recommended for immunofluorescent analysis of human samples. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human IRF-5 protein. Background Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). Alternate Names Interferon regulatory factor 5; IRF-5; IRF5; SLEB10 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG