CST, 43890SF, CD63 (D4I1X) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD63. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CD63 (D4I1X) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #43890) is ready to ship. Product Usage Information This product is the carrier free version of product #55051. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 55051 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD63 (D4I1X) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD63 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant CD63 protein. Background CD63 belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2) (1-3). Tetraspanins interact with a variety of cell surface proteins and intracellular signaling molecules in specialized tetraspanin-enriched microdomains (TEMs) where they mediate a range of processes, including adhesion, motility, membrane organization, and signal transduction (3). CD63, like other tetraspanins, is enriched in exosomes (4). It is also a component of Weibel-Palade bodies found in endothelial cells (5). Research studies demonstrate several functions of CD63 in different cell types, including roles in mast cell degranulation, VEGF signaling in endothelial cells, recruitment of leukocytes to endothelial cells, and endosomal sorting during melanogenesis (6-9). Alternate Names CD63; CD63 antigen; CD63 antigen (melanoma 1 antigen); CD63 molecule; Granulophysin; LAMP-3; Lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; ME491; melanoma 1 antigen; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; Ocular melanoma-associated antigen; OMA81H; Tetraspanin-30; Tspan-30; TSPAN30 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG