CST, 43918S, MTH1 (D6V4O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NUDT1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MTH1 (D6V4O) Rabbit Monoclonal Antibody (CST #43918) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MTH1 (D6V4O) Rabbit Monoclonal Antibody recognizes endogenous levels of total MTH1 protein. Based on the sequence of the immunogenic peptide, this antibody is expected to recognize all isoforms of MTH1. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala70 of human MTH1 protein. Background MutT Homolog 1 (MTH1), an oxidized purine nucleoside triphosphatase, hydrolyzes potentially mutagenic oxidized nucleotide triphosphates, preventing their accumulation in nucleotide pools and their incorporation into DNA and RNA (1). In addition to its function in sanitizing the cell's nucleotide pool, MTH1 has been shown to have anti-proliferative effects in RAS-transformed tumors (2). Researchers have shown that, while not essential in normal cells, MTH1 is required for cancer cell survival due to increased oxidative damage, and that inhibition of MTH1 activity suppresses cancer growth (3,4). Alternate Names 2-hydroxy-dATP diphosphatase; 7,8-dihydro-8-oxoguanine triphosphatase; 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase; 8-oxo-7,8-dihydroguanosine triphosphatase; 8-oxo-dGTPase; 8ODP; MTH1; mutT human homolog 1; Nucleoside diphosphate-linked moiety X motif 1; nucleoside diphosphate-linked moiety X-type motif 1; nudix (nucleoside diphosphate linked moiety X)-type motif 1; nudix hydrolase 1; Nudix motif 1; nudix-type motif 1; NUDT1; Oxidized purine nucleoside triphosphate hydrolase Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG