CST, 4402T, FoxP1 (D35D10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FOXP1. Validated for WB,IP,IHC,F,ChIP,C&R,C&T. Available in 2 sizes. Highly specific and rigorously validated in-house, FoxP1 (D35D10) Rabbit Monoclonal Antibody (CST #4402) is ready to ship. Product Usage Information For optimal ChIP or ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 . Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 Chromatin IP: 1:100 CUT&RUN: 1:50 CUT&Tag: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 85650 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized), Chromatin IP, CUT&RUN, CUT&Tag Specificity / Sensitivity FoxP1 (D35D10) Rabbit Monoclonal Antibody detects endogenous levels of total FoxP1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu584 of human FoxP1 protein. Background Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5). FoxP1 plays an important role in cardiac valve and B cell development. Targeted deletion of FoxP1 in mice results in embryonic death at E14.5 with severe defects in cardiac outflow development, endocardial cushion morphogenesis, and myocyte proliferation and maturation (6). The FoxP1 gene is located on chromosome 3p14.1 and loss of heterozygosity of this region in multiple cancer types suggests a role for FoxP1 as a tumor suppressor (7). Alternatively, FoxP1 may also act as an oncogene as it is highly expressed in a variety of B cell malignancies and is frequently involved in chromosome translocation (8,9). Alternate Names 12CC4; FLJ23741; fork head-related protein like B; forkhead box P1; Forkhead box protein P1; FOXP1; glutamine-rich factor 1; hFKH1B; HSPC215; Mac-1-regulated forkhead; MFH; MGC12942; MGC88572; MGC99551; QRF1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 82-90 Source/Isotype: Rabbit IgG