CST, 44073SF, YKL-40 (E2L1M) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying YKL-40. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, YKL-40 (E2L1M) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #44073) is ready to ship. Product Usage Information This product is the carrier free version of product #47066. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 47066 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity YKL-40 (E2L1M) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total YKL-40 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human YKL-40 protein. Background YKL-40, also known as chitinase-3-like protein 1 (CHI3L1), is a secreted glycoprotein encoded by the gene (1,2). Unlike other members of the chitinase-like protein family, YKL-40 is not an active chitinase. It is produced by activated macrophages, chondrocytes, neutrophils, and synovial cells. It is a pro-inflammatory molecule that contributes to the progression of many inflammatory diseases, including fibrosis, neurodegenerative disease, breast, lung, prostate, liver, bladder, colon, and other types of cancers (3,4). Levels of YKL-40 in serum and plasma in patients with various types of tumors have been reported to predict poor prognosis, and in some clinical assays, YKL-40 is being used as a predictive biomarker of cancer outcome (4). Alternate Names 39 kDa synovial protein; ASRT7; Cartilage glycoprotein 39; CGP-39; CH3L1; CHI3L1; chitinase 3 like 1; chitinase 3-like 1; chitinase 3-like 1 (cartilage glycoprotein-39); Chitinase-3-like protein 1; DKFZp686N19119; FLJ38139; GP-39; GP39; HC-gp39; hCGP-39; HCGP-3P; YK-40; YKL-40; YKL40; YYL-40 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30-40 Source/Isotype: Rabbit IgG