CST, 4420S, NAC1 Antibody

Polyclonal Antibody for studying BTBD14B. Validated for Western Blotting. Highly specific and rigorously validated in-house, NAC1 Antibody (CST #4420) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NAC1 Antibody detects endogenous levels of total NAC1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to an amino acid sequence at the carboxyl terminus of human NAC1. Antibodies are purified by protein A and peptide affinity chromatography. Background NAC1 or nuclear accumbens-1 is a nuclear factor that belongs to the POZ/BTB (Pox virus and zinc finger/bric-a-brac tramtrack broad complex) domain family. Also known as BTBD14B, it was originally identified in a unique neuronal forebrain structure responsible for reward motivation and addictive behaviors (1,2). NAC1 recruits HDAC3 and HDAC4 to transcriptionally repress gene expression in neuronal cells (3) and specifically co-represses other POZ/BTB proteins in the central nervous system (4). NAC1 is upregulated in several tumor types, including breast, renal cell, and hepatocellular carcinoma, as well as high grade ovarian serous carcinoma, where it has long been suspected as a chemoresistance gene (5,6). The chemoresistance mechanism reportedly occurs through NAC1 negative regulation of the GADD45 pathway (7). NAC1 has also been described as part of the extended transcriptional network in pluripotent cells that involves Oct-4, Sox2, Nanog, Sall1, KLF4 and Sall4 (8). Alternate Names BEN domain containing 8; BEND8; BTB (POZ) domain containing 14B; BTB/POZ domain-containing protein 14B; BTBD14B; BTBD30; FLJ37383; NAC-1; NAC1; NACC1; NECFM; nucleus accumbens associated 1; nucleus accumbens associated 1, BEN and BTB (POZ) domain containing; Nucleus accumbens-associated protein 1; transcriptional repressor NAC1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 62 SOURCE: Rabbit