CST, 4422S, Paip2A Antibody

Polyclonal Antibody for studying Paip2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Paip2A Antibody (CST #4422) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Paip2A Antibody detects endogenous levels of total Paip2A protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human Paip2A. Antibodies are purified by protein A and peptide affinity chromatography. Background The eukaryotic mRNA 3' poly(A) tail interacts with the 5' cap structure to regulate translation of mRNA transcripts (1). The eIF4G translation initiation factor associates with poly(A) binding protein (PABP) to circularize mRNA (1,2). Paip1 is a PABP interacting protein that binds eIF4A to stimulate translation (3). A second PABP binding protein, Paip2, inhibits translation by interfering with PABP function. Specifically, Paip2 reduces the affinity of PABP for poly(A) RNA and competes with Paip1 for PABP binding (1,2). Alternate Names MGC72018; PABC1-interacting protein 2; PABP-interacting protein 2; PAIP-2; PAIP2; PAIP2A; poly(A) binding protein interacting protein 2; Poly(A)-binding protein-interacting protein 2; polyA-binding protein-interacting protein 2; Polyadenylate-binding protein-interacting protein 2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 25 SOURCE: Rabbit