CST, 4426T, FIH (D19B3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FIH. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, FIH (D19B3) Rabbit Monoclonal Antibody (CST #4426) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FIH (D19B3) Rabbit Monoclonal Antibody detects endogenous levels of total FIH protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr35 of human FIH protein. Background FIH (Factor inhibiting HIF-1, HIF asparagine hydroxylase) is a dioxygen-dependent asparaginyl hydroxylase that modifies target protein function by hydroxylating target protein asparagine residues (1-3). Hypoxia-inducible factor (HIF), a transcriptional activator involved in control of cell cycle in response to hypoxic conditions, is an important target for FIH regulation. FIH functions as an oxygen sensor that regulates HIF function by hydroxylating at Asn803 in the carboxy-terminal transactivation domain (CAD) of HIF (4,5). During normoxia, FIH uses cellular oxygen to hydroxylate HIF-1 and prevent interaction of HIF-1 with transcriptional coactivators, including the CBP/p300-interacting transactivator. Under hypoxic conditions, FIH remains inactive and does not inhibit HIF, allowing the activator to regulate transcription of genes in response to low oxygen conditions (4-6). FIH activity is regulated in through interaction with proteins, including Siah-1, which targets FIH for proteasomal degradation (7). The Cut-like homeodomain protein CDP can bind the FIH promoter region to regulate FIH expression at the transcriptional level (8). Phosphorylation of HIF at Thr796 also can prevent FIH hydroxylation on Asn803 (9). Potential FIH substrates also include proteins with ankyrin repeat domains, such as Iκ-B, Notch, and ASB4 (10-12). Alternate Names DKFZp762F1811; Factor inhibiting HIF-1; factor inhibiting HIF1; FIH-1; FIH1; FLJ20615; FLJ22027; HIF1AN; HIF1N; hypoxia inducible factor 1 alpha subunit inhibitor; hypoxia inducible factor 1 subunit alpha inhibitor; hypoxia inducible factor 1, alpha subunit inhibitor; Hypoxia-inducible factor 1-alpha inhibitor; Hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG