CST, 44305SF, HEF1/NEDD9 (2G9) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying NEDD9. Validated for Western Blotting. Highly specific and rigorously validated in-house, HEF1/NEDD9 (2G9) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #44305) is ready to ship. Product Usage Information This product is the carrier free version of product #4044. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 4044 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity HEF1/NEDD9 (2G9) Mouse Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total HEF1/NEDD9 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment corresponding to residues from the human HEF1/NEDD9 protein. Background Human enhancer of filamentation protein 1 (HEF1), also known as neural precursor cell expressed developmentally down-regulated protein 9 (NEDD9), is part of the Cas family of proteins, which include HEF1/NEDD9, p130Cas and Efs (1). HEF1 is a predominantly cytoplasmic protein, localizing to focal adhesions during interphase, and centrosomes and other parts of the mitotic apparatus during G2/M phase of the cell cycle (2). HEF1 is a docking protein that plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion, motility, growth and apoptosis (1). Phosphorylation of HEF1 is induced by a number of factors, including FAK, TGF-β, PDGFR, Abl, and BCR-ABL, which leads to coordinate binding of multiple downstream effector proteins via 15 known SH2 domain-binding sites (1). HEF1 is a key regulator of cancer metastasis. It is required for the invasive activity of glioblastomas (3), is linked to the promotion of metastasis in melanoma (4), and is found to be up-regulated in lung cancer metastasis (5). Alternate Names Cas scaffolding protein family member 2; CAS-L; cas-like docking; CAS2; CASL; CASS2; Crk-associated substrate related; Crk-associated substrate related protein Cas-L; CRK-associated substrate-related protein; dJ49G10.2; dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)); dJ761I2.1; dJ761I2.1 (enhancer of filamentation (HEF1)); Enhancer of filamentation 1; Enhancer of filamentation 1 p55; hEF1; NEDD-9; NEDD9; Neural precursor cell expressed developmentally down-regulated protein 9; neural precursor cell expressed, developmentally down-regulated 9; p105; p130Cas-related protein; Renal carcinoma antigen NY-REN-12 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 105, 115 Source/Isotype: Mouse IgG1