CST, 44339S, PU.1 (F2D5A) Mouse Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying PU.1 mouse. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, PU.1 (F2D5A) Mouse Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #44339) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity PU.1 (F2D5A) Mouse Monoclonal Antibody (Alexa Fluor Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu27 of mouse PU.1 protein. Background PU.1 is a member of the Ets family of transcription factors and activates target genes through the purine-rich PU-box (1). PU.1 plays a pivotal role in the differentiation of myeloid cells and lymphocytes and is expressed in several hematopoietic cells, including B lymphocytes, macrophages, neutrophils, mast cells, early erythroid cells, and megakaryocytes (1,2). The concentration of PU.1 is critical for both the determination of hematopoietic cell lineage and the regulation of differentiation versus stem cell proliferation (3,4). In addition, PU.1 activity is influenced by phosphorylation and interactions with other hematopoietic transcription factors. Phosphorylation of PU.1 at Ser146 by CK2 promotes binding to IRF-4 and synergistic activation through the immunoglobulin κ 3' enhancer (5). Treatment of pro-B cells with IL-3 leads to phosphorylation of PU.1 at Ser140, resulting in increased PU.1 activity and activation of the anti-apoptotic gene (6). GATA1 binding blocks PU.1 activity during erythroid cell development (7). Overexpression of PU.1 resulting from proviral insertion during Friend virus infection can induce erythroleukemia, while reduced expression has been associated with acute myeloid leukemia (8). Alternate Names 31 kDa-transforming protein; Dis; Dis-1; Dis1; hematopoietic transcription factor PU.1; OTTMUSP00000015228; PU.; PU.1; SFFV proviral integration 1; SFFV proviral integration 1 protein; Sfp; Sfpi-1; Sfpi1; Spi-1; Spi1; spleen focus forming virus (SFFV) proviral integration oncogene; Tcfpu; Tcfpu1; Tfpu.; Tfpu.1; Transcription factor PU.1 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1 kappa