CST, 44353S, CYFIP1 Antibody

Polyclonal Antibody for studying CYFIP1. Validated for Western Blotting. Highly specific and rigorously validated in-house, CYFIP1 Antibody (CST #44353) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CYFIP1 Antibody recognizes endogenous levels of total CYFIP1 protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro527 of human CYFIP1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Cytoplasmic FMR1-interacting protein 1 (CYFIP1) is a component of the CYFIP1/EIF4E/FMR1 complex which mediates translational repression by binding to the mRNA cap (1). CYFIP1 also plays a role in neuronal axonal growth dynamics by binding to the WAVE complex to regulate remodeling of actin filaments (2). Mutations in the gene encoding CYFIP1 has been linked to multiple neural development and psychiatric disorders, including autism spectrum disorder and schizophrenia (3-6). The specific mechanism by which CYFIP1, which is enriched in synapses, contributes to these neurological diseases is unknown, but may involve regulating the balance of synaptic excitation and inhibition to maintain neuronal circuit homeostasis during development and in mature brains (7). Alternate Names CYFIP1; CYFP1; cytoplasmic FMR1 interacting protein 1; Cytoplasmic FMR1-interacting protein 1; cytoplasmic FMRP interacting protein 1; FLJ45151; KIAA0068; p140sra-1; selective hybridizing clone; SHYC; Specifically Rac1-associated protein 1; Sra-1; SRA1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 145 SOURCE: Rabbit