CST, 4435S, CARD11 (1D12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CARD11. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CARD11 (1D12) Rabbit Monoclonal Antibody (CST #4435) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CARD11 (1D12) Rabbit Monoclonal Antibody detects endogenous levels of total CARD11 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding threonine 175 of CARD11. Background CARD11/Carma1/Bimp3 belongs to the MAGUK (membrane-associated guanylate kinase) family that typically function as molecular scaffolds in the assembly of multiprotein complexes (1,2). MAGUK family members contain an SH3 domain, a PDZ domain and a GuK domain homologous to guanylate kinase. In addition, CARD11 contains an amino-terminal CARD domain (caspase recruitment domain). This domain plays an important role in forming interactions with a number of proteins containing CARD domains that are involved in regulating apoptosis and NF-κB activation. CARD11 is predominately expressed in lymphocytes (1,2) and associates with the CARD domain of Bcl10. When overexpressed, CARD11 leads to the phosphorylation of Bcl10 and activation of NF-κB (1,2). CARD11 is constitutively associated with lipid rafts and is thought to function by recruiting Bcl10 and MALT1 and triggering the phosphorylation of IKKs (3,4). Several studies using the genetic disruption of CARD11 or dominant-negative mutations have demonstrated that it plays a critical role in NF-κB activation and lymphocyte signaling (4-7). Alternate Names bcl10-interacting maguk protein 3; BENTA; BIMP3; CAR11; CARD-containing MAGUK protein 1; card-maguk protein 1; CARD11; Carma 1; CARMA1; caspase recruitment domain family member 11; caspase recruitment domain family, member 11; Caspase recruitment domain-containing protein 11; IMD11; IMD11A; MGC133069; PPBL Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG