CST, 4437S, TWEAK Antibody

Polyclonal Antibody for studying TNFSF12. Validated for Western Blotting. Highly specific and rigorously validated in-house, TWEAK Antibody (CST #4437) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TWEAK Antibody detects recombinant and transfected levels of TWEAK protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His110 of human Tweak protein. Antibodies were purified by protein A and peptide affinity chromatography. Background TWEAK (TNFSF12/Apo-3L) is a member of the TNF superfamily of cytokines that are typically involved in immune regulation, inflammation, and apoptosis (1,2). TWEAK mRNA is expressed in a variety of tissues and cell lines, with higher levels observed in the heart, brain, skeletal muscle and within the immune system (1). Like other family members TWEAK is a type II transmembrane protein that can also be proteolytically processed to form a soluble cytokine. Soluble TWEAK is a weak inducer of apoptosis in some cell lines (1). The receptor for TWEAK, known as TWEAKR or fibroblast growth factor inducible 14 (Fn14), is a relatively small member of the TNF receptor family (3). TWEAK signaling has been associated with apoptosis, proliferation, migration, angiogenesis, and inflammation (4). Recent studies have suggested some therapeutic potential of TWEAK and its receptor signaling in regards to autoimmunity, cancer, and vascular injury (5-8). Alternate Names APO3 ligand; APO3/DR3 ligand; APO3L; DR3LG; MGC129581; MGC20669; TNF superfamily member 12; TNF-related weak inducer of apoptosis; TNF12; TNFSF12; TNLG4A; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand 4A; Tumor necrosis factor ligand superfamily member 12; Tumor necrosis factor ligand superfamily member 12, membrane form; Tumor necrosis factor ligand superfamily member 12, secreted form; tumor necrosis factor superfamily member 12; TWEAK Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 30 SOURCE: Rabbit