CST, 44427S, Phospho-c-Myb (Ser11) (F6D5K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying c-Myb (Ser11) phosphate. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-c-Myb (Ser11) (F6D5K) Rabbit Monoclonal Antibody (CST #44427) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Phospho-c-Myb (Ser11) (F6D5K) Rabbit Monoclonal Antibody recognizes endogenous levels of c-Myb protein only when phosphorylated at Ser11. This antibody does not cross-react with a-Myb or b-Myb proteins. This antibody will detect all c-Myb protein isoforms containing the Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser11 of human c-Myb protein. Background c-Myb is a transcriptional activator that specifically recognizes the sequence 5'-YAAC[GT]G-3'. It is expressed in hematopoietic progenitor cells, where it plays an important role in the control of proliferation and differentiation (1-3). c-Myb is required for transcription of genes involved in self-renewal of intestinal stem cells. Importantly, c-Myb regulates expression of Lgr5, a protein expressed in putative intestinal stem cells that give rise to all cell lineages of small intestinal crypts (4). c-Myb is reported to be expressed in colon crypt cells and in human colorectal cancer cell lines (5,6). Research has shown that gene translocations and copy number alterations are found in several leukemias, breast cancer, and other solid tumors (7,8). The c-Myb DNA-binding domain (DBD) can be phosphorylated at Ser11/Ser12 by casein kinase 2 (CK2), which similarly phosphorylates a-Myb and b-Myb, while it is uniquely phosphorylated by protein kinase A (PKA) (9,10). Ser11/Ser12 phosphorylation reduces c-Myb transactivation to target genes and alters its interaction with the cooperative transcription factor C/EBP beta (10). These inhibitory sites are frequently deleted in c-Myb-driven cancers (11), and c-Myb isoforms lacking the DBD are aberrantly expressed in adenoid cystic carcinomas through an alternative promoter (12). Alternate Names c-myb; c-myb protein (140 AA); c-myb_CDS; c-myb10A_CDS; c-myb13A_CDS; c-myb14A_CDS; c-myb8B_CDS; Cmyb; efg; MYB; MYB proto-oncogene, transcription factor; oncogene AMV; Proto-oncogene c-Myb; Transcriptional activator Myb; v-myb avian myeloblastosis viral oncogene homolog; v-myb myeloblastosis viral oncogene homolog (avian) Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 80-95 Source/Isotype: Rabbit IgG