CST, 44446S, Bax (E4U1V) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying Bax. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Bax (E4U1V) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #44446) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Bax (E4U1V) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala42 of human Bax protein. Background Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9, and initiation of the caspase activation pathway for apoptosis (3,4). Alternate Names Apoptosis regulator BAX; BAX; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; Bcl-2-like protein 4; BCL2 associated X protein; BCL2 associated X, apoptosis regulator; BCL2-associated X protein; BCL2-associated X protein omega; Bcl2-L-4; BCL2L4 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG