CST, 4450S, Caspase-4 Antibody

Polyclonal Antibody for studying CASP4. Validated for Western Blotting. Highly specific and rigorously validated in-house, Caspase-4 Antibody (CST #4450) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Caspase-4 Antibody detects endogenous levels of total caspase-4 protein. Processing intermediate forms of caspase-4 are observed at 40 kDa and 32 kDa as previously reported (7). This antibody does not cross-react with other caspases. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile125 within the p20 subunit of human caspase-4 protein. Antibodies were purified by protein A and peptide affinity chromatography. Background Caspase-4 (TX/ICH-2/ICE II) is a member of the caspase family of proteases that play a key role in the execution of apoptosis and activation of inflammatory cytokines (1-3). Expression of caspase-4 has been observed in most tissues except brain, with highest levels in placenta, lung, spleen, and peripheral blood lymphocytes (PBL). Caspase-4 was originally found to contribute to Fas-mediated apoptosis (4). Several caspases (including caspase-4, caspase-5, and mouse caspase-11 and -12) are most closely related to caspase-1 and are capable of inducing apoptosis when overexpressed but are better characterized in the proteolytic activation of inflammatory cytokines (5). Caspase-4 associates with TRAF6 and is involved in the LPS inducible production of inflammatory cytokines IL-8 and MIP1 in THP-1 cells (6). While caspase-4 and mouse caspase-12 localize to the endoplasmic reticulum (ER) and may be activated by drugs that induce ER stress (7), at least one study suggests that caspase-4 and caspase-12 are not essential for ER stress-induced apoptosis (8). Alternate Names apoptotic cysteine protease Mih1/TX; CASP-4; CASP4; caspase 4; caspase 4, apoptosis-related cysteine peptidase; caspase 4, apoptosis-related cysteine protease; Caspase-4; Caspase-4 subunit 1; Caspase-4 subunit 2; Caspase4; ICE and Ced-3 homolog 2; ICE(rel)-II; ICE(rel)II; ICEREL-II; ICH-2; ICH2; Mih1; Mih1/TX; Protease ICH-2; Protease TX; TX Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45 SOURCE: Rabbit