CST, 44570S, FBXL10 (D3T8J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FBXL10. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, FBXL10 (D3T8J) Rabbit Monoclonal Antibody (CST #44570) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FBXL10 (D3T8J) Rabbit Monoclonal Antibody recognizes transfected levels of total FBXL10 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the human FBXL10 protein. Background F-box and leucine-rich repeat protein 10 (FBXL10), also known as lysine-specific demethylase 2B (KDM2B) and JmjC domain-containing histone demethylation protein 1B (JHDM1B), is a lysine-specific histone demethylase protein that demethylates Lys4 and Lys36 of histone H3 (1,2). FBXL10 contains a zinc finger-CxxC5 DNA binding domain that binds to un-methylated CpG dinucleotides and has been shown to function as part of a non-canonical polycomb repressor complex (PRC1) that is associated with repression of developmentally regulated genes (3-5). In embryonic stem cells, FBXL10 is critical for targeting PRC1 to CpG islands and regulating gene expression during differentiation. In addition, FBXL10 is over-expressed in various cancers where it functions to induce cell proliferation and repress senescence through repression of the p15Ink4b gene locus (6-8). High expression of FBXL10 in pancreatic ductal adenocarcinoma is associated with metastasis, while high expression in acute myeloid leukemia is associated with increased proliferation and self-renewal of leukemic stem cells (6-8). Alternate Names [Histone-H3]-lysine-36 demethylase 1B; CXXC-type zinc finger protein 2; CXXC2; F-box and leucine-rich repeat protein 10; F-box protein FBL10; F-box/LRR-repeat protein 10; FBL10; FBXL10; JEMMA (Jumonji domain, EMSY-interactor, methyltransferase motif) protein; JHDM1B; JmjC domain-containing histone demethylation protein 1B; jumonji C domain-containing histone demethylase 1B; Jumonji domain-containing EMSY-interactor methyltransferase motif protein; KDM2B; lysine (K)-specific demethylase 2B; lysine demethylase 2B; Lysine-specific demethylase 2B; NDY1; PCCX2; protein containing CXXC domain 2; Protein JEMMA; Protein-containing CXXC domain 2 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 180 Source/Isotype: Rabbit IgG