CST, 44579T, dCK (F1F7B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying dCK mouse. Validated for WB,WB,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, dCK (F1F7B) Rabbit Monoclonal Antibody (CST #44579) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity dCK (F1F7B) Rabbit Monoclonal Antibody recognizes endogenous levels of total dCK protein. Reactivity for immunofluorescence and flow cytometry is mouse only. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys252 of mouse dCK protein. Background Deoxycytidine kinase (dCK) catalyzes the rate-limiting step in the nucleoside salvage pathway. dCK phosphorylates salvaged deoxycytidine (dC) to generate deoxycytidine monophosphate (dCMP), a precursor for both deoxycytidine triphosphate (dCTP) and deoxythymidine triphosphate (dTTP) pools (1). dCK also phosphorylates deoxyadenosine (dA) and deoxyguanosine (dG) to generate dA monophosphate and dG monophosphate, respectively. Gemcitabine is an anticancer nucleoside that is a dC analog. It is a pro-drug and must be phosphorylated by dCK to an active form. dCK is phosphorylated on Ser74 by the DNA damage signaling kinases ATM and ATR, and this phosphorylation increases dCK activity (2,3). Alternate Names Dck; Deoxyadenosine kinase; Deoxycytidine kinase; Deoxyguanosine kinase; OTTMUSP00000030586 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rabbit IgG