CST, 44585S, Crry Antibody

Polyclonal Antibody for studying Crry mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, Crry Antibody (CST #44585) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Crry Antibody recognizes endogenous levels of total Crry protein. Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu99 of mouse Crry protein. Antibodies are purified by peptide affinity chromatography. Background Crry, also known as complement component receptor 1-like protein (Cr1l), is a murine membrane-bound complement regulatory protein ubiquitously expressed in mouse tissues (1,2). Crry is a functional analog of the human complement decay-accelerating factor (DAF/CD55) and membrane cofactor protein (MCP/CD46) (3). In mice, Crry acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissues (4,5). Crry also acts as a decay-accelerating factor, preventing the formation of C4b2a and C3bBb, which are amplification convertases of the complement cascade (3,6). Crry plays a crucial role in early embryonic development by maintaining fetomaternal tolerance (7). On CD4 T cells, Crry acts as a costimulatory factor which favors IL-4 rather than IFN-gamma secretion, suggesting Th2 polarization (8). Cancer cells and cells of the tumor microenvironment have been shown to overexpress Crry, suppressing tumor-specific T cell responses and complement-mediated anti-tumor immunity (9,10). Multiple isoforms have been identified computationally. Alternate Names complement component (3b/4b) receptor 1-like; Complement component receptor 1-like protein; complement receptor related protein; Complement regulatory protein Crry; Cr1l; Crry; Cry; Mcp; mCR; mCRY; MGC102484; Protein p65 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 60-80 SOURCE: Rabbit