CST, 44665S, XCR1 (D2F8T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying XCR1. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, XCR1 (D2F8T) Rabbit Monoclonal Antibody (CST #44665) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63684 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity XCR1 (D2F8T) Rabbit Monoclonal Antibody recognizes endogenous levels of total XCR1 protein by IHC-P and transfected levels of XCR1 protein by Western. Staining of unknown specificity in peripheral nerves has been observed. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human XCR1 protein. Background X-C motif chemokine receptor 1 (XCR1, GPR5, CCXCR1), part of the G protein-coupled superfamily, is expressed by a subset of dendritic cells and acts as a receptor for chemokines XCL1 and XCL2 (1,2,4). XCR1-positive dendritic cells cross-present antigens to naïve CD8 T cells, priming them to become activated cytotoxic CD8 T cells (3-5). In mouse models, the XCL1-XCR1 signaling axis was shown to be involved in the formation of self-tolerance through the development of T cells within the thymus (6). Alternate Names CCXCR1; chemokine (C motif) receptor 1; chemokine (C motif) XC receptor 1; Chemokine XC receptor 1; G protein-coupled receptor 5; G-protein coupled receptor 5; GPR5; Lymphotactin receptor; X-C motif chemokine receptor 1; XC chemokine receptor 1; XCR1 Specification REACTIVITY: H SENSITIVITY: Endogenous (IHC-P), Transfected (W) MW (kDa): 35 Source/Isotype: Rabbit IgG