CST, 4467S, Bif-1 Antibody

Polyclonal Antibody for studying Bif-1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Bif-1 Antibody (CST #4467) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Bif-1 Antibody recognizes endogenous levels of total Bif-1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly129 of human Bif-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Bif-1/SH3GLB1/Endophilin-B1 is a member of the endophilin B family that was originally identified as a Bax binding protein through yeast two-hybrid screening (1,2). Bif-1 does not have significant homology to other Bcl-2 family members, but rather contains an N-terminal Bin-Amphiphysin-Rvs (BAR) domain, typically involved in membrane dynamics, and a C-terminal SH3 domain. Overexpression of Bif-1 promotes Bax conformational change and apoptosis (2,3). Likewise, loss of Bif-1 inhibits Bax and Bak activation, cytochrome c release, and caspase activation (3). Bif-1 is localized to membranes of intracellular organelles and has been suggested to play a role in membrane dynamics, including that during autophagy. Bif-1 directly binds to UVRAG, forming a complex with Beclin-1, resulting in increased PI3-kinase class III/Vps34 activity required for autophagosome maturation (4). Inhibition of GSK-3β, as seen during nutrient deprivation, results in increased expression of Bif-1, and can contribute to autophagic cell death (5). Research studies have shown that loss of Bif-1 promotes tumorigenesis, and decreased expression of Bif-1 has been noted in several cancer types (4,6-10). Alternate Names Bax-interacting factor 1; Bif-1; CGI-61; dJ612B15.2; endophilin B1; Endophilin-B1; KIAA0491; PPP1R70; protein phosphatase 1, regulatory subunit 70; SH3 domain containing GRB2 like, endophilin B1; SH3 domain-containing GRB2-like protein B1; SH3-containing protein SH3GLB1; SH3-domain GRB2 like endophilin B1; SH3-domain GRB2-like endophilin B1; SH3GLB1; SHLB1; testicular tissue protein Li 172 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 42 SOURCE: Rabbit