CST, 4470T, RagD Antibody

Polyclonal Antibody for studying RagD. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, RagD Antibody (CST #4470) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RagD Antibody detects endogenous levels of total RagD protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser37 of human RagD protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The mTORC1 kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). RagD is one of several genes differentially expressed in human melanoma cell lines and has been considered as a viable target for further diagnostic and therapeutic study (5). Alternate Names bA11D8.2.1; DKFZp761H171; FLJ44176; Rag D; Rag D protein; RagD; Ras related GTP binding D; Ras-related GTP binding D; Ras-related GTP-binding protein D; RRAGD Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 SOURCE: Rabbit