CST, 44717S, SMARCAL1 (D3P5I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SMARCAL1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SMARCAL1 (D3P5I) Rabbit Monoclonal Antibody (CST #44717) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SMARCAL1 (D3P5I) Rabbit Monoclonal Antibody recognizes endogenous levels of total SMARCAL1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala933 of human SMARCAL1 protein. Background SMARCAL1 was first identified as a ubiquitously expressed member of the SNF2 family with homology to the protein HepA (1). Mutations in the gene encoding SMARCAL1 were subsequently shown to be the cause of Schimke immuno-osseous dysplasia (SIOD), an autosomal recessive disorder characterized by phenotypes in multiple systems, including spondyloepiphyseal dysplasia, renal dysfunction, immunodeficiency, and impaired neurological function (2). Researchers have also associated SMARCAL1 deficiency with predisposition to non-Hodgkin's lymphoma (3). The array of phenotypes associated with SMARCAL1 is likely due to its role as an annealing helicase in the DNA damage response. During DNA replication stress, SMARCAL1 is phosphorylated by DNA repair kinases (ATM, ATR, DNA-PK) (4). SMARCAL1 deficiency sensitizes cells to replication stress agents, and appears to increase the frequency of replication fork breakdown (4,5). SMARCAL1 is also required for efficient DNA double strand break repair via the nonhomologous end joining (NHEJ) DNA repair pathway (6). Researchers have suggested that inhibitors targeting SMARCAL1 may be effective in sensitizing cancer cells to chemotherapeutic agents (reviewed in 7). Alternate Names ATP-driven annealing helicase; HARP; HepA-related protein; hHARP; SMAL1; SMARCA-like protein 1; SMARCAL1; Sucrose nonfermenting protein 2-like 1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a like 1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 105 Source/Isotype: Rabbit IgG