CST, 4472S, HPK1 Antibody

Polyclonal Antibody for studying HPK1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, HPK1 Antibody (CST #4472) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity HPK1 Antibody detects endogenous levels of total HPK1. This antibody does not cross-react with other members of the GCK family. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of human HPK1. Antibodies are purified by protein A and peptide affinity chromatography. Background Hematopoietic progenitor kinase 1 (HPK1) is a member of the germinal center kinases that is predominantly expressed in hematopoietic cells. HPK1 is a key component linking T or B cell receptors to SAPK/JNK and IkappaB kinase pathways in lymphocytes (1-3). Through its proline-rich motif, HPK1 associates with multiple SH3 domain-containing adaptor proteins, including GRB2, Nck, Crk, SLP-76, and BLNK. Phosphorylation of Tyr379 by Syk is necessary for HPK1 association with BLNK and SLP-76, as well as for HPK1 activity (4,5). Caspase cleavage of HPK1 also modulates the biological function of HPK1 (3,6,7). Alternate Names Hematopoietic progenitor kinase; hematopoietic progenitor kinase 1; HPK1; M4K1; MAP4K1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; Mitogen-activated protein kinase kinase kinase kinase 1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 97 SOURCE: Rabbit