CST, 4475S, Scribble Antibody

Polyclonal Antibody for studying Scribble. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Scribble Antibody (CST #4475) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Scribble Antibody detects endogenous levels of total scribble protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid residues surrounding Gly1237 of human scribble protein. Antibodies are purified by protein A and peptide affinity chromatography Background Scribble (Scrib) was originally identified in a genetic screen in along with cell polarity determinants Discs Large (Dlg) and Lethal giant larvae (Lgl). mutants homozygous for these genes share similar phenotypes, including the loss of apicobasal cell polarity and neoplastic tissue overgrowth. These phenotypic similarities suggest that these three proteins function in a common pathway important for establishing and maintaining apicobasal polarity in epithelial cells (1,2). Scribble contains many leucine-rich repeats and PDZ domains important for localizing scribble to adherens junctions and basolateral regions of mammalian epithelial cells (3). Scribble reportedly binds β-catenin, APC, E-cadherin and the E6 protein from high-risk virus type of HPV through a short motif important for E6-induced cell transformation (4-8). Overexpression of scribble inhibits transformation of rodent epithelial cells by HPV E6/7 proteins (8). Alternate Names CRIB1; hScrib; KIAA0147; LAP4; Protein LAP4; Protein scribble homolog; SCRB1; SCRIB; SCRIB1; Scribble; scribble planar cell polarity protein; scribbled homolog; scribbled homolog (Drosophila); scribbled planar cell polarity protein; VARTUL Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 240 SOURCE: Rabbit