CST, 44774S, LAG3 (D2G4O™) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying Lag3. Validated for Western Blotting. Highly specific and rigorously validated in-house, LAG3 (D2G4O^™) Rabbit Monoclonal Antibody (Biotinylated) (CST #44774) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LAG3 (D2G4O™) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total LAG3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human LAG3 protein. Background Lymphocyte activation gene 3 (LAG3, CD223) is an immune checkpoint control protein that negatively regulates T cells and immune responses. A CD4-like member of the Ig superfamily, LAG3 contains an extracellular IgV and three IgC domains, a transmembrane domain, and a short cytoplasmic region (1). LAG3 is primarily expressed by activated CD4 T cells, CD8 T cells, Tregs, and NK cells, where it's activated by MHC Class II molecules, its only known ligand. While it was initially shown to activate Treg cells (2), LAG3 can also inhibit CD8 T cells (3,4). LAG3 is often co-expressed with PD-1 on the surface of tumor infiltrating lymphocytes, where the two proteins act independently to contribute to tumor-mediated immune suppression (4,5). Blockade of LAG3 is a promising strategy for neoplastic intervention (6). Alternate Names CD223; FDC; LAG-3; LAG3; lymphocyte activating 3; Lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Protein FDC; Secreted lymphocyte activation gene 3 protein; sLAG-3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60-80 Source/Isotype: Rabbit IgG