CST, 44786S, NRCAM (D2H1T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NRCAM. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, NRCAM (D2H1T) Rabbit Monoclonal Antibody (CST #44786) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NRCAM (D2H1T) Rabbit Monoclonal Antibody recognizes endogenous levels of total human NRCAM protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of the human NRCAM protein. Background Neuronal Cell Adhesion Molecule, or NRCAM, belongs to the immunoglobulins Cell Adhesion Molecules (CAM's) superfamily (1). NRCAM, an ankyrin-binding protein, contributes to the neurite outgrowth by providing directional signaling during axonal cone growth (2, 3, 4). Additionally, it plays a role in mediating the interaction between axons and Schwann cells and contributes to the formation and maintenance of Nodes of Ranvier (5, 6, 7, 8). NRCAM also plays an important role in the establishment of dendritic spines in developing cortical neurons (9). NRCAM is expressed in non-neuronal cells, mostly in endothelial cells (10). Alternate Names hBravo; KIAA0343; MGC138845; MGC138846; Neuronal cell adhesion molecule; Neuronal surface protein Bravo; Ng-CAM-related; NgCAM-related cell adhesion molecule; Nr-CAM; NRCAM; NrCAM bravo Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG