CST, 44800SF, Fibronectin/FN1 (E5H6X) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying FN1. Validated for WB,IHC,IHC,IF. Highly specific and rigorously validated in-house, Fibronectin/FN1 (E5H6X) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #44800) is ready to ship. Product Usage Information This product is the carrier free version of product #26836. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 26836 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Fibronectin/FN1 (E5H6X) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Fibronectin/FN1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a protein fragment corresponding to residues near the carboxy terminus of human Fibronectin/FN1 protein. Background Fibronectin is a multi-domain extracellular matrix (ECM) protein. The protein uses its different domains to bind distinct ECM components such as collagens, growth factors, and cell surface integrins to carry on its functions (1). Fibronectin has been implicated in many essential biological processes, including tissue repair, fibrosis, and tumor development (1,2). There are many fibronectin isoforms. Plasma fibronectin is synthesized by hepatocytes and exists as a compact, inactive conformation in the bloodstream. It is a major component of fibrin clots. Upon binding to integrins or other cell surface receptors, fibronectin switches to an extended conformation, and exposes its function domains to activated extracellular matrix assembly (3). Fibroblasts, endothelial cells, and many types of cancer cells have also been shown to synthesize cellular fibronectin isoforms. Among them, the EDA or EDB isoforms are particularly highly expressed during fibrosis and cancer development, and are potential diagnostic and therapeutic targets (4-6). Alternate Names Anastellin; CIG; Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED-B; epididymis secretory sperm binding protein; Fibronectin; fibronectin 1; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; migration-stimulating factor; MSF; SMDCF; Ugl-Y1; Ugl-Y2; Ugl-Y3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 300 Source/Isotype: Rabbit IgG