CST, 44813S, Rab27B Antibody

Polyclonal Antibody for studying RAB27B. Validated for Western Blotting. Highly specific and rigorously validated in-house, Rab27B Antibody (CST #44813) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Rab27B Antibody recognizes endogenous levels of total Rab27B protein. Overexpression analysis confirms that this antibody does not detect Rab27A. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Rab27B protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Rab27 proteins are members of the Ras superfamily of small Rab GTPases associated with the process of exocytosis (1-2), and in particular the exosome secretion pathway (3-5). There are two Rab27 isoforms, Rab27A and Rab27B, which are encoded by separate genes and which exhibit distinct subcellular localization and functions. Rab27A colocalizes to CD63 vesicles, whereas Rab27B is observed in perinuclear vesicles. Targeted knockdown studies suggest that the isoforms control distinct steps of the exosome secretion pathway (6). Rab27A participates in docking and membrane fusion from multivesicular endosomes (MVEs) to exosomes, whereas Rab27B mediates the transfer of membrane vesicles from the trans-Golgi network to MVEs. It has been suggested that Rab27A and Rab27B may have importance in exosome secretion by cancer cells (7). Alternate Names C25KG; RAB27B; RAB27B, member RAS oncogene family; Ras-related protein Rab-27B; RB27B Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 27 SOURCE: Rabbit