CST, 4482S, THEMIS Antibody

Polyclonal Antibody for studying THEMIS. Validated for Western Blotting. Highly specific and rigorously validated in-house, THEMIS Antibody (CST #4482) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity THEMIS Antibody recognizes endogenous levels of total THEMIS protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human THEMIS protein. Antibodies are purified by protein A and peptide affinity chromatography. Background THEMIS is a recently identified protein found to be critical for T cell development (1-5). It contains two amino-terminal globular domains, a nuclear localization signal, and a carboxy-terminal proline-rich sequence with homology to SH3 binding domains (2,3). THEMIS is detected in both the cytoplasm and the nucleus (2). It is expressed at low levels in mature T cells and during thymocyte development, with expression peaking in CD4 CD8 double positive cells (1-5). THEMIS is tyrosine phosphorylated downstream of TCR signaling and associates with the adaptor GRB2 and possibly other SH3 domain-containing proximal TCR signaling molecules (1-5). Mice lacking THEMIS have a defect in positive selection that results in decreased numbers of both single positive thymocytes and mature T cells (1-5). Alternate Names bA325O24.3; bA325O24.4; C6orf190; C6orf207; FLJ40584; GASP; GRB2-associated protein; MGC163388; Protein THEMIS; signaling phosphoprotein specific for T cells; SPOT; THEMIS; THMS1; thymocyte selection associated; thymocyte selection pathway associated; Thymocyte-expressed molecule involved in selection; TSEPA Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 74 SOURCE: Rabbit