CST, 44865S, Perforin (E9F7N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Perforin mouse. Validated for WB,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Perforin (E9F7N) Rabbit Monoclonal Antibody (CST #44865) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:50 - 1:100 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Perforin (E9F7N) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse perforin protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to mouse perforin protein. Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3). Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells (4). Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells. Alternate Names Cytolysin; Lymphocyte pore-forming protein; P1; PERF; perforin 1 (pore forming protein); Perforin-1; Pfn; Pfp; pore forming protein; Prf; Prf-1; Prf1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 70-75 Source/Isotype: Rabbit IgG