CST, 44873S, Filamin A (E8P8M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Filamin A. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Filamin A (E8P8M) Rabbit Monoclonal Antibody (CST #44873) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Frozen): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 72246 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity Filamin A (E8P8M) Rabbit Monoclonal Antibody recognizes endogenous levels of total filamin A protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central sequence of human filamin A protein. Background Filamins are a family of dimeric actin binding proteins that function as structural components of cell adhesion sites. They also serve as a scaffold for subcellular targeting of signaling molecules (1). The actin binding domain (α-actinin domain) located at the amino terminus is followed by as many as 24 tandem repeats of about 96 residues and the dimerization domain is located at the carboxy terminus. In addition to actin filaments, filamins associate with other structural and signaling molecules such as β-integrins, Rho/Rac/Cdc42, PKC and the insulin receptor, primarily through the carboxy-terminal dimerization domain (1-3). Filamin A, the most abundant, and filamin B are widely expressed isoforms, while filamin C is predominantly expressed in muscle (1). Filamin A is phosphorylated by PAK1 at Ser2152, which is required for PAK1-mediated actin cytoskeleton reorganization (4). Alternate Names ABP-280; ABPX; actin binding protein 280; Actin-binding protein 280; Alpha-filamin; CSBS; CVD1; DKFZp434P031; Endothelial actin-binding protein; epididymis secretory sperm binding protein; FGS2; filamin 1; filamin A; filamin A, alpha; Filamin-1; Filamin-A; FLN; FLN-A; FLN1; FLNA; FMD; MNS; NHBP; Non-muscle filamin; OPD; OPD1; OPD2; XLVD; XMVD Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 280 Source/Isotype: Rabbit IgG