CST, 4497S, NIPA Antibody

Polyclonal Antibody for studying NIPA. Validated for Western Blotting. Highly specific and rigorously validated in-house, NIPA Antibody (CST #4497) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NIPA Antibody detects endogenous levels of total NIPA protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with synthetic peptide corresponding to the amino acid sequence surrounding Ala130 of human NIPA. Antibodies are purified by protein A and peptide affinity chromatography. Background NIPA (nuclear interaction partner of ALK) is an F-box-containing protein that is an essential component of the SCF-type E3 ligase (SCF ) complex, a complex that controls the completion of S-phase and mitotic entry (1). This control is mediated by the ubiquitination and subsequent degradation of cell cycle regulatory proteins, whose oscillation of protein levels is required for proper cell cycle progression (2). Expression levels of NIPA are low in G0/G1 phases and upregulated in S and G2/M phases. The SCF complex targets nuclear cyclin B1 for ubiquitination in interphase, whereas phosphorylation of NIPA in late G2 phase and mitosis inactivates the complex to allow for accumulation of cyclin B1 (3). NIPA may have an anti-apoptotic role in NPM-ALK-mediated signaling events (4). Alternate Names 1110054L24Rik; hematopoietic stem/progenitor cell protein 216; hNIPA; HSPC216; NIPA; nuclear interacting partner of anaplastic lymphoma kinase (ALK); nuclear interaction partner of ALK; Nuclear-interacting partner of ALK; Nuclear-interacting partner of anaplastic lymphoma kinase; ZC3HC1; zinc finger C3HC-type containing 1; Zinc finger C3HC-type protein 1; zinc finger, C3HC type 1; zinc finger, C3HC-type containing 1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 60-65 SOURCE: Rabbit