CST, 4502T, IRS-2 Antibody

Polyclonal Antibody for studying IRS-2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, IRS-2 Antibody (CST #4502) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IRS-2 Antibody detects endogenous levels of total IRS-2 protein. This antibody does not cross-react with IRS-1. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of Ser1282 of human IRS-2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3). Alternate Names Insulin receptor substrate 2; IRS-2; IRS2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 185 SOURCE: Rabbit