CST, 4504S, IRAK1 (D51G7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IRAK1. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, IRAK1 (D51G7) Rabbit Monoclonal Antibody (CST #4504) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 48222 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity IRAK1 (D51G7) Rabbit Monoclonal Antibody detects endogenous levels of total IRAK1 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of mouse IRAK1. Background Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3). Alternate Names interleukin 1 receptor associated kinase 1; Interleukin-1 receptor-associated kinase 1; IRAK; IRAK-1; IRAK1; pelle; Pelle homolog Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 78-105 Source/Isotype: Rabbit IgG