CST, 4505S, TAK1 Antibody

Polyclonal Antibody for studying TAK1. Validated for Western Blotting. Highly specific and rigorously validated in-house, TAK1 Antibody (CST #4505) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TAK1 Antibody detects endogenous levels of total TAK1 protein. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of human TAK1. Antibodies are purified by protein A and peptide affinity chromatography. Background TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein, and other cytokines, including IL-1 (1,2). activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2). Alternate Names CSCF; FMD2; M3K7; MAP3K7; MEKK7; Mitogen-activated protein kinase kinase kinase 7; TAK1; TGF-beta activated kinase 1; TGF-beta-activated kinase 1; TGF1a; Transforming growth factor-beta-activated kinase 1 Specification REACTIVITY: H M R Mk B SENSITIVITY: Endogenous MW (kDa): 78 to 82 SOURCE: Rabbit