CST, 45071SF, Spinophilin (E1E7R) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Spinophilin. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Spinophilin (E1E7R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #45071) is ready to ship. Product Usage Information This product is the carrier free version of product #14136. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 14136 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Spinophilin (E1E7R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total spinophilin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro298 of human spinophilin protein. Background Spinophilin is an 815 amino acid protein composed of a PDZ domain, 2 actin-binding domains, a receptor- and PP1-binding domain, three coiled-coiled domains, a potential leucine/isoleucine zipper motif, and three potential SH3 domains (1). Spinophilin interacts with a large number of proteins including ion channel components and G protein-coupled receptors (GPCRs). Spinophilin also interacts with actin filaments; phosphorylation of spinophilin at Ser94 and Ser177 disrupts this interaction (2). Spinophilin has been shown to affect GPCR function through two different mechanisms: spinophilin acts as a functional inhibitor of α-2 adrenergic receptor-mediated arrestin signaling by competing with GRK2 binding to the adrenergic receptor (3) and spinophilin facilitates μ-opioid receptor desensitization by promoting receptor endocytosis (4). Alternate Names FLJ30345; NEB2; neurabin II; Neurabin-2; Neurabin-II; PPP1R6; PPP1R9; PPP1R9B; Protein phosphatase 1 regulatory subunit 9B; protein phosphatase 1, regulatory (inhibitor) subunit 9B; protein phosphatase 1, regulatory subunit 9B; protein phosphatase 1, regulatory subunit 9B, spinophilin; SPINO; Spinophilin; SPL; Spn Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG