CST, 4508T, Phospho-TAK1 (Thr184/187) (90C7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TAK1 (Thr184/Thr187) phosphate. Validated for Western Blotting,Simple Western™. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-TAK1 (Thr184/187) (90C7) Rabbit Monoclonal Antibody (CST #4508) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-TAK1 (Thr184/187) (90C7) Rabbit Monoclonal Antibody detects endogenous levels of TAK1 only when phosphorylated at threonine 184 and threonine 187. In some cell lysates, this antibody may cross-react with a 40 kDa band of unknown origin. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a phosphopeptide corresponding to residues surrounding Thr184 and Thr187 of human TAK1. Background TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein, and other cytokines, including IL-1 (1,2). activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2). Alternate Names CSCF; FMD2; M3K7; MAP3K7; MEKK7; Mitogen-activated protein kinase kinase kinase 7; TAK1; TGF-beta activated kinase 1; TGF-beta-activated kinase 1; TGF1a; Transforming growth factor-beta-activated kinase 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 82 Source/Isotype: Rabbit IgG