CST, 45159S, SV40 Large T Antigen (D1E9E) Rabbit Monoclonal Antibody (Sepharose Bead Conjugate)

Monoclonal Antibody for studying SV40 large T antigen. Validated for Immunoprecipitation. Highly specific and rigorously validated in-house, SV40 Large T Antigen (D1E9E) Rabbit Monoclonal Antibody (Sepharose Bead Conjugate) (CST #45159) is ready to ship. Product Usage Information Immunoprecipitation: 1:20 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunoprecipitation Specificity / Sensitivity SV40 Large T Antigen (D1E9E) Rabbit Monoclonal Antibody (Sepharose Bead Conjugate) recognizes transgenic levels of total SV40 Large T Antigen protein. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys315 of SV40 Large T Antigen protein. Background Simian Virus 40 (SV40) is a small double-stranded, circular DNA tumor virus encoding three tumor antigens: large T, Small T, and 17KT. These antigens contribute to cellular transformation, an area of research where they are utilized extensively. Transformation of cells by SV40 large T antigen is mediated by its binding to the tumor suppressor proteins RB and p53 in the nucleus. Additional binding partners include p300, CBP, Cul7, IRS1, Bub1, Nbs1, and Fbw7, which also contribute to transformation (1). Alternate Names Large T antigen; LT; lT-AG; simian virus 40 large T antigen; SV40 large T antigen Specification REACTIVITY: All SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG