CST, 45262S, Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) Monoclonal Antibody

Secondary Antibody for studying in the research area. Product Usage Information Recommended Antibody Dilution: 1:2000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) Monoclonal Antibody recognizes the light chain of rabbit IgG, either in its native conformation or in its denatured and reduced form. This antibody does not bind to the denatured and reduced rabbit IgG heavy chain (about 50 kDa) on western blot. Species Reactivity: Rabbit Source / Purification Monoclonal antibody is produced by immunizing animals with native total rabbit IgG. Background Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb only reacts with the light chain of rabbit IgG and does not bind to the denatured and reduced rabbit IgG heavy chain. When performing immunoprecipitation (IP) followed by western blotting, the denatured rabbit IgG heavy chain of the primary antibody used for IP runs at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have a similar molecular weight. Using Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb as a secondary antibody will eliminate this problem. Specification REACTIVITY: Rab Source/Isotype: Mouse