CST, 45348S, CD83 (F9D2B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD83 mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CD83 (F9D2B) Rabbit Monoclonal Antibody (CST #45348) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CD83 (F9D2B) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD83 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse CD83 protein. Background CD83 is a single-transmembrane protein with a calculated molecular weight (MW) of 23 kDa, but due to heavy and differential glycosylation, its apparent MW ranges from 23 to 70 kDa. CD83 is predominantly expressed on mature dendritic cells (DCs) and has been used as a DC activation/maturation marker as its increased expression is correlated with upregulation of HLA class II antigen expression on DCs (1-3). CD83 is also expressed at a low level on lymphocytes and is upregulated upon lymphocyte activation (4,5). Thymic epithelial cells also express CD83, which is required for normal CD4 T cell development (6,7). CD83 is also expressed as a soluble form (sCD83) that can be found in serum of healthy adults (8). sCD83 has been shown to negatively regulate immune response by lymphocytes (9,10). Alternate Names Cd83; CD83 antigen; mCD83; MGC130312; MGC130313 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 23-65 Source/Isotype: Rabbit IgG