CST, 4536S, Phospho-TAK1 (Thr187) Antibody

Polyclonal Antibody for studying TAK1 (Thr187) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-TAK1 (Thr187) Antibody (CST #4536) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-TAK1 (Thr187) Antibody detects endogenous levels of TAK1 only when phosphorylated at threonine 187. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr187 of human TAK1. Antibodies are purified by protein A and affinity chromatography. Background TAK1 is a mitogen-activated protein kinase kinase kinase that can be activated by TGF-β, bone morphogenetic protein, and other cytokines, including IL-1 (1,2). activation of TAK1 requires association with TAK1 binding protein 1 (TAB1), which triggers phosphorylation of TAK1 (3,4). Another adaptor protein, TAB2, links TAK1 with TRAF6 and mediates TAK1 activation upon IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK, respectively. In addition, TAK1 activates the NF-κB pathway by interacting with TRAF6 and phosphorylating the NF-κB inducing kinase (NIK) (2). TAK1 activation requires multiple phosphorylations in its activation loop. Mutation of Thr187 and Thr184, residues located in the activation loop of TAK1, impairs phosphorylation of both TAK1 and TAB1 and reduces the kinase activity of TAK1, suggesting that autophosphorylation of these residues is necessary for TAK1 activation (4). Alternate Names CSCF; FMD2; M3K7; MAP3K7; MEKK7; Mitogen-activated protein kinase kinase kinase 7; TAK1; TGF-beta activated kinase 1; TGF-beta-activated kinase 1; TGF1a; Transforming growth factor-beta-activated kinase 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 82 SOURCE: Rabbit