CST, 45389S, SETDB1 (F6H2L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SETDB1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SETDB1 (F6H2L) Rabbit Monoclonal Antibody (CST #45389) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SETDB1 (F6H2L) Rabbit Monoclonal Antibody recognizes endogenous levels of total SETDB1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro713 of human SETDB1 protein. Background The Erg-associated protein with SET domain (ESET), also known as SET-domain, bifurcated 1 (SETDB1) protein, is a member of a histone lysine methyltransferase family, each of which contains a conserved catalytic SET domain, originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). SETDB1 also contains tudor and methyl-CpG-binding domains, which may coordinate binding to methylated histones and methylated DNA, respectively (1). SETDB1 methylates histone H3 Lys9, creating a transcriptionally repressive mark that facilitates gene silencing (1-3). However, unlike SUV39H histone H3 Lys9 methyltransferases, which function mainly in heterochromatin regions, such as pericentric heterochromatin, SETDB1 functions mainly in euchromatic regions to repress gene promoters (3). SETDB1 interacts with a variety of proteins, including transcription factors (ERG), histone deacetylases (HDAC1/2), DNA methyltransferases (DNMT3A/B) and transcriptional co-repressors (mSin3A/B, MBD1, KAP-1, the ATFa-associated modulator mAM) (1-6). mAM forms a complex with SETDB1, stimulating its methyltransferase activity, specifically the conversion of di-methyl to tri-methyl histone H3 Lys9 (2). MBD1 recruits SETDB1 to the CAF-1 complex to facilitate methylation of histone H3 Lys9 during replication-coupled chromatin assembly in S phase (5). DNMT3A recruits SETDB1 to silenced promoters in cancer cells (7). SETDB1 may play a role in the pathogenesis of Huntington's disease, since levels of SETDB1 protein and tri-methyl histone H3 Lys9 are both increased in diseased brains (8). Alternate Names ERG-associated protein with a SET domain, ESET; ERG-associated protein with SET domain; ESET; H3-K9-HMTase 4; H3-K9-HMTase4; Histone H3-K9 methyltransferase 4; Histone-lysine N-methyltransferase SETDB1; histone-lysine N-methyltransferase, H3lysine-9 specific 4; KG1T; KIAA0067; KMT1E; Lysine N-methyltransferase 1E; SET domain bifurcated 1; SET domain bifurcated histone lysine methyltransferase 1; SET domain, bifurcated 1; SETB1; SETDB1; TDRD21; tudor domain containing 21 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 180 Source/Isotype: Rabbit IgG