CST, 4538S, MUC1 (VU4H5) Mouse Monoclonal Antibody

Monoclonal Antibody for studying MUC1. Validated for WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, MUC1 (VU4H5) Mouse Monoclonal Antibody (CST #4538) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:100 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 68123 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity MUC1 (VU4H5) Mouse Monoclonal Antibody detects endogenous levels of total MUC1. This antibody does not cross-react with other mucin proteins. Species Reactivity: Human Source / Purification Monoclonal antibody (isotype: IgG1k) is produced by immunizing a BALB/c mouse with MUC1 60 mer tandem repeats (BSA conjugated). Background Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation (1). MUC1 (or mucin 1) is aberrantly overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a 72-residue cytoplasmic tail (1). MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation (2). MUC1 also interacts with β-catenin, which is regulated by GSK-3β, PKCγ, and Src through phosphorylation at Ser44, Thr41, and Tyr46 of the MUC1 cytoplasmic tail (3-5). Overexpression of MUC1 potentiates transformation (6) and attenuates stress-induced apoptosis through the Akt or p53 pathways (7,8). Alternate Names ADMCKD; ADMCKD1; Breast carcinoma-associated antigen DF3; CA 15-3; Cancer antigen 15-3; Carcinoma-associated mucin; CD227; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; KL-6; Krebs von den Lungen-6; MAM6; MCD; MCKD; MCKD1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1; MUC1-alpha; MUC1-beta; MUC1-CT; MUC1-NT; MUC1/ZD; mucin 1, cell surface associated; mucin 1, transmembrane; Mucin-1; Mucin-1 subunit alpha; Mucin-1 subunit beta; Peanut-reactive urinary mucin; PEM; PEMT; Polymorphic epithelial mucin; PUM; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 230, 400 Source/Isotype: Mouse IgG1